MCQs on Biotechnology

MCQs on Biotechnology

1. Restriction enzymes were discovered by
a. Smith and Nathans
b. Alexander Fleming
c. Berg
d. None

2. Bacteria protect themselves from viruses by fragmenting viral DNA with
a. Ligase
b. Endonuclease
c. Exonuclease
d. Gyrase

3. Klenow fragment is derived from
a. DNA Ligase
b. DNA Pol-I
c. DNA Pol-II
d. Reverse Transcriptase

4. Southern blotting is
a. Attachment of probes to DNA fragments
b. Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet
c. Comparison of DNA fragments to two sources
d. Transfer of DNA fragments to electrophoretic gel from cellulose membrane

5. ELISA is
a. Using radiolabelled second antibody
b. Usage of RBCs
c. Using complement-mediated cell lysis
d. Addition of substrate that is converted into a coloured end product

6. The Golden Rice variety is rich in
a. Vitamin C
b. Β-carotene and ferritin
c. Biotin
d. Lysine

7. The DNA fragments have sticky ends due to
a. Endonuclease
b. Unpaired bases
c. Calcium ions
d. Free methylation

8. Plasmids are used as cloning vectors for which of the following reasons?
a. Can be multiplied in culture
b. Self-replication in bacterial cells
c. Can be multiplied in laboratories with the help of enzymes
d. Replicate freely outside bacterial cells

9. The human genome project was launched in the year
a. 1980
b. 1973
c. 1990
d. 1989

10. The vaccines prepared through recombinant DNA technology are
a. Third generation vaccines
b. First-generation vaccines
c. Second-generation vaccines
d. None

11. Which is a genetically modified crop?
a. Bt-cotton
b. Bt-brinjal
c. Golden rice
d. All

12. PCR technique was invented by
a. Karry Mullis
b. Boyer
c. Sanger
d. Cohn

13. The first transgenic plant to be produced is
a. Brinjal
b. Tobacco
c. Rice
d. Cotton

14. RNA interference helps in
a. Cell proliferation
b. Micropropagation
c. Cell defence
d. Cell differentiation

15. Which of the following is the quality of improved transgenic basmati rice?
a. Gives high yield but no characteristic aroma
b. Gives high yield and is rich in vitamin A
c. Does not require chemical fertilizers and growth hormones
d. Resistant to insects and diseases

16. The first clinical application of gene therapy over a 4 year old girl was for
a. Adenosine deaminase deficiency
b. Adenosine deficiency
c. Growth deficiency
d. Adenine deficiency

17. Excision and insertion of a gene is called
a. Biotechnology
b. Genetic engineering
c. Cytogenetics
d. Gene therapy

18. The expression of a transgene in the target tissue is identified by a
a. Transgene
b. Promoter
c. Enhancer
d. Reporter

19. ———– is used as a vector for cloning into higher organisms
a. Retrovirus
b. Baculovirus
c. Salmonella typhimurium
d. Rhizopus nigricans

20. Which bacterium is used in the production of insulin by genetic engineering?
a. Saccharomyces
b. Rhizobium
c. Escherichia
d. Mycobacterium

Answer key